Maarten van der Doelen

Immunophenotyping during radium-223 therapy in mCRPC patients

Supplementary table 1. Antibody details. Antigen Clone

Conjugate

Company

Catalog No.

Dilution

Panel 1 (T cell panel) CD3

UCHT1

BV605 BV510

Biolegend

300460 659454 565310 562443 560608 557301 560816

1:40 1:25 1:10 1:40 1:25 1:10

CD4 CD8

SK3 SK1

BD biosciences BD biosciences BD biosciences BD biosciences BD biosciences BD biosciences

PerCP-Cy5.5

CD25

M-A251 UCHL1

BV421 PE-Cy7

CD45RO CTLA-4

BNI3

PE

CCR7 FoxP3

150503 PCH101

AF647

1:5

3

A488

eBioscience

53-4776-42

1:10

Panel 2 (Checkpoint panel 1) CD3 UCHT1

PE-Cy7 BV510 BV605

eBioscience

25-0038-42

1:25 1:25 1:40 1:10

CD4 CD8

SK3

BD Biosciences BD Biosciences eBioscience BioLegend BD Bioscience

659454 743066

G42-8

LAG-3 PD-L1 ICOS PD-1 TIM-3

3DS223H 29E.2A3

FITC APC

11-2239-42

329708 562833 329920 345006 300460 659454 565310 555837 557301 558017 329708 353906 555339 562723 560827 560827 345792 303408 562794 558066 558017 563083 F0768

1:5

DX29

PerCP-Cy5.5

1:10

EH12.2H7

BV421

BioLegend BioLegend

1:5

F38-2E2

PE

1:10

Panel 3 (Checkpoint panel 2) CD3 UCHT1

BV605 BV510

Biolegend

1:40 1:25 1:10

CD4 CD8

SK3 SK1

BD Biosciences BD Biosciences BD biosciences

PerCP-Cy5.5

OX-40 CTLA-4

ACT35

FITC

1:5

BNI3 MIH1

PE

Biosciences Biosciences BioLegend Biolegend

1:10

PD-L1 PD-1 TIM-1

PE-Cy7 BV421

1:5 1:5

EH12.2H7

1D12

APC

1:25

Panel 4 (MDSC panel) CD3

HIT3α ICRF44 MΦP9

FITC

BD Biosciences BD Biosciences BD Biosciences BD Biosciences

1:10 1:10 1:10 1:10 1:10 1:10 1:10 1:10

CD11b CD14 CD15 CD19 CD20 CD33 CD56 PD-L2 PD-L1 HLA-DR

BV605 BV421

HI98

PerCP-Cy5.5

HD37

FITC FITC APC FITC

Agilent

L27

BD Biosciences

WM53

Biolegend

B159

BD Biosciences BD Biosciences BD Biosciences BD Biosciences

MIH18 MIH1 G46-6

PE

1:5 1:5

PE-Cy7 BV510

1:25

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