Maarten van der Doelen

Chapter 3

CTLA-4

ICOS

PD1

PD-L1

TIM-1

TIM-3

12 15 18

150 200 250 300

0 30 60

0 500 1000

30

400

20

CD8 + cells CD4 + cells

300

6 9

10

200

10 15 20 25 30 35

0 100 200 300

20 40

200 300 400 500

∆MFI

− 100 0 100

2500

− 20 − 40 0

− 2500 0

BL 2 4 6

BL 2 4 6

BL 2 4 6

BL 2 4 6

BL 2 4 6

BL 2 4 6

Injection number

Supplementary figure 4. Analysis of the ∆MFI of checkpoint molecules on CD4 + and CD8 + T cells. ∆MFI values are normalized. Red dots indicate the group mean. The blue line represents the fitted linear regression model, including 95% CI. CI, confidence interval; ∆MFI, median fluorescence intensity of the test sample minus the median fluorescence intensity of the negative fluorescence minus one control .

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